Food Science

CLO (Clorprenaline) ELISA Kit (FSES0061)

(No reviews yet) Write a Review
SKU:
FSES0061
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science

Description

Technical Manual

CLO (Clorprenaline) ELISA Kit

Kit Name: CLO (Clorprenaline) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can be used to detect Clorprenaline (CLO) in samples, such as serum, urine, tissue, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, CLO in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CLO antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of CLO. The concentration of CLO in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.05 ppb (ng/mL)
Reaction Mode: 25°C,30 min~15 min
Detection Limit: Serum --- 0.25 ppb; urine, Tissue --- 0.5 ppb
Cross Reactivity:

Clorprenaline

100%

Tulobuterol 

91%

Dobutamine, Mabuterol, Ractopamine

<0.1%

Clenproperol, Cimaterol, Cimbuterol 

<0.1%

Clenpenterol, Mapenterol, Terbutaline

<0.1%

Bromchlorbuterol, Salbutamol, Clenbuterol

<0.1%

Brombuterol, Carbuterol

<0.1%

Sample Recovery Rate: 100%±30%.
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1.5 mL each (0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05ppb)
Sample Dilution 30 mL
11×Concentrated HRP Conjugate Solution 1 mL
HRP Conjugate Dilution 10 mL
Substrate Reagent A 7 mL
Substrate Reagent B 7 mL
Stop Solution 7 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate Reader, Homogenizer, water bath, Oscillators, Centrifuge, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Contact Technical Support
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
View AllClose

0 Reviews

View AllClose