Metabolism Assays
Citrate Assay Kit (Colorimetric or Fluorometric) (BA0090)
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- SKU:
- BA0090
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Detection Method:
- Fluorometric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Tissue, Cells
- Research Area:
- Ferroptosis
- Research Area:
- TCA Cycle
- Research Area:
- Food Safety & Analysis
- Research Area:
- Plant & Environmental Stress
- Research Area:
- Cation and Inorganic Ion
- Research Area:
- Clinical Chemistry
Description
Citrate Assay Kit - Information
Assay Genie's Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the citrate concentration in the sample.Applications
For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.Citrate Assay Kit - Key Features
- Fast and sensitive. Linear detection range: 4 to 400 citrate for colorimetric assays and 0.5 to 40 for fluorimetric assays.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Citrate Assay Kit - Data Sheet | |
| Kit Includes | Developer: 10 mL CL Enzyme: Dried Dye Reagent: 120 mL ODC Enzyme: 120 mL Citrate Standard: 500 mL |
| Kit Requires | Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes. |
| Method of Detection | OD570nm, or FL530/585nm |
| Detection Limit | OD, FL: 4, 0.5 . |
| Samples | Serum, plasma, agriculture etc |
| Species | All |
| Protocol Length | 20 min |
| Size | 100 tests |
| Storage | Store all kit components at -20 °C |
| Shelf Life | 6 months |
More Details
CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis.Related Products
