Citrate Assay Kit (Colorimetric or Fluorometric) (BA0090)

Citrate Assay Kit - Information

Assay Genie's Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the citrate concentration in the sample.

Applications

For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.

Citrate Assay Kit - Key Features

• Fast and sensitive. Linear detection range: 4 to 400 citrate for colorimetric assays and 0.5 to 40 for fluorimetric assays.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Citrate Assay Kit - Data Sheet
Kit Includes	Developer: 10 mL CL Enzyme: Dried Dye Reagent: 120 mL ODC Enzyme: 120 mL Citrate Standard: 500 mL
Kit Requires	Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.
Method of Detection	OD570nm, or FL530/585nm
Detection Limit	OD, FL: 4, 0.5 .
Samples	Serum, plasma, agriculture etc
Species	All
Protocol Length	20 min
Size	100 tests
Storage	Store all kit components at -20 °C
Shelf Life	6 months

More Details

CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis.