Cholinesterase (CHE) Activity Assay Kit

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SKU:
MAES0210
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Animal Tissue
Sensitivity:
2.17 U/mL
Range:
2.17-71.33 U/mL
Assay Time:
60 min

Description

Product Name:Cholinesterase (CHE) Activity Assay Kit
Product Code:MAES0210
Sample Type:Serum, Plasma, Animal Tissue
Assay Time:60 min
Assay Type:Enzyme Activity
Synonyms:CHE
Detection Instrument:Microplate reader (505-535 nm, optimum wavelength: 520 nm)
Detection Method:Colorimetric method
Sensitivity:2.17 U/mL
Detection Range:2.17-71.33 U/mL
Inter-assay CV:0.064
Intra-assay CV:0.024

Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.

Storage:2-8°C
Valid Period:12 months
Other Reagents Required:Normal saline (0.9% NaCl)
Other Instruments Required:EP tube, centrifuge, Vortex mixer, 37℃ water bath, Microplate reader
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