Metabolism Assays

Choline Assay Kit (BA0088)

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SKU:
BA0088
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Saliva, Milk, Tissue, Cells
Research Area:
Food Safety & Analysis
Research Area:
Plant & Environmental Stress
Research Area:
Clinical Chemistry

Description

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Choline Assay Kit - Information

Assay Genie's choline assay provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.

Applications

For quantitative determination of choline and evaluation of drug effects on choline metabolism.

Choline Assay Kit - Key Features

  • Use 20 samples. Linear detection range: colorimetric assay 1 to 100 , fluorimetric assay 0.2 to 10 choline.

Choline Assay Kit - Data Sheet
Kit Includes Assay Buffer: 10 mL Enzyme Mix: Dried Dye Reagent: 120 uL Standard: 400 uL 2 mM Choline
Kit Requires Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader
Method of Detection OD570nm, or FL530/585nm
Detection Limit OD, FL: 1, 0.2
Samples Serum, plasma, urine, saliva, milk, tissue, cells etc
Species All
Protocol Length 30 min
Size 100 tests
Storage Store all components at -20°C
Shelf Life 6 months

More Details

CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.

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