Name: Cholesterol Assay Kit (Colorimetric) (BA0084)
Brand: Metabolism Assays
SKU: BA0084
Availability: OutOfStock

Cholesterol Assay Kit (Colorimetric) (BA0084)

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SKU:

BA0084

Product Type:

Assay

Detection Method:

Colorimetric

Instrument:

Microplate Reader

Sample Type:

Serum, Plasma

Research Area:

Ferroptosis

Research Area:

Lipid Metabolism

Research Area:

Diabetes & Obesity

Research Area:

Food Safety & Analysis

Research Area:

Clinical Chemistry

Description
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Description

Cholesterol Assay Kit - Information

Assay Genie's colorimetric cholesterol assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene-3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

Applications

For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.

Cholesterol Assay Kit - Key Features

Sensitive and accurate. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.
Convenient. Room temperature assay. No 37°C heater is needed.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Cholesterol Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 20 mL Enzyme Mix: 120 uL NAD Solution: 2 x 1 mL Standard: 1 mL 300mg/dL cholesterol
Kit Requires	Pipetting (multi-channel) devices, clear bottom 96-well plate and plate reader.
Method of Detection	OD340nm
Detection Limit	5 mg/dL
Samples	Serum, plasma etc
Species	All
Protocol Length	40 min
Size	100 tests
Storage	Store reagents at -20°C
Shelf Life	6 months

More Details

CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable.