CHML Antibody (PACO63635)
- SKU:
- PACO63635
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | CHML Antibody (PACO63635) |
| Antibody SKU: | PACO63635 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Rab proteins geranylgeranyltransferase component A 2 protein (480-656AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of U251 cells with PACO63635 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family. |
| Synonyms: | Rab proteins geranylgeranyltransferase component A 2 (Choroideremia-like protein) (Rab escort protein 2) (REP-2), CHML, REP2 |
| UniProt Protein Function: | CHML: Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than REP-1 in supporting prenylation of Rab3 family. Belongs to the Rab GDI family.Chromosomal Location of Human Ortholog: 1q43Cellular Component: cytoplasm; nucleoplasm; Rab-protein geranylgeranyltransferase complexMolecular Function: Rab GTPase bindingBiological Process: protein geranylgeranylation |
| UniProt Protein Details: | |
| NCBI Summary: | The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P26374 |
| NCBI GenInfo Identifier: | 47117837 |
| NCBI Gene ID: | 1122 |
| NCBI Accession: | P26374.2 |
| UniProt Secondary Accession: | P26374,Q17RE0, Q9H1Y4, B2RAB9 |
| UniProt Related Accession: | P26374 |
| Molecular Weight: | 74,071 Da |
| NCBI Full Name: | Rab proteins geranylgeranyltransferase component A 2 |
| NCBI Synonym Full Names: | CHM like, Rab escort protein 2 |
| NCBI Official Symbol: | CHML |
| NCBI Official Synonym Symbols: | REP2 |
| NCBI Protein Information: | rab proteins geranylgeranyltransferase component A 2 |
| UniProt Protein Name: | Rab proteins geranylgeranyltransferase component A 2 |
| UniProt Synonym Protein Names: | Choroideremia-like protein; Rab escort protein 2; REP-2 |
| Protein Family: | Rab proteins geranylgeranyltransferase component |
| UniProt Gene Name: | CHML |
| UniProt Entry Name: | RAE2_HUMAN |
| Antibodies |
| CHML Antibody (PACO01418) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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