Antibody Name:	CFL1/CFL2 Antibody (PACO22037)
Antibody SKU:	PACO22037
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Synthesized peptide derived from internal of human Cofilin.

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot analysis of extracts from Jurkat cells and HeLa cells, using Cofilin antibody.

Background:

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

Synonyms:

CFL2; COF2; Cofilin 2; Cofilin; muscle isoform

UniProt Protein Function:	Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; intercellular junction; membrane; nuclear matrix; nucleus; vesicle Molecular Function:actin binding; protein binding Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction
NCBI Summary:	The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
UniProt Code:	P23528
NCBI GenInfo Identifier:	116848
NCBI Gene ID:	1072
NCBI Accession:	P23528.3
UniProt Secondary Accession:	P23528,Q53Y87, Q9UCA2, B3KUQ1,
UniProt Related Accession:	P23528
Molecular Weight:	18,502 Da
NCBI Full Name:	Cofilin-1
NCBI Synonym Full Names:	cofilin 1
NCBI Official Symbol:	CFL1
NCBI Official Synonym Symbols:	CFL; cofilin; HEL-S-15
NCBI Protein Information:	cofilin-1
UniProt Protein Name:	Cofilin-1
UniProt Synonym Protein Names:	18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
Protein Family:	Cofilin
UniProt Gene Name:	CFL1
UniProt Entry Name:	COF1_HUMAN