Antibody Name:	CENPU Antibody (PACO16695)
Antibody SKU:	PACO16695
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:	Human
Immunogen:	Fusion protein of human CENPU

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO16695(CENPU Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO16695(CENPU Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).

Background:

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly.

Synonyms:

centromere protein U

UniProt Protein Function:	CENPU: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 4q35.1Cellular Component: cytosol; microtubule organizing center; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: DNA replication-independent nucleosome assembly at centromere; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:	The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q71F23
NCBI GenInfo Identifier:	74712714
NCBI Gene ID:	79682
NCBI Accession:	Q71F23.1
UniProt Secondary Accession:	Q71F23,Q09GN2, Q32Q71, Q9H5G1, A2RRD9
UniProt Related Accession:	Q71F23
Molecular Weight:	38,102 Da
NCBI Full Name:	Centromere protein U
NCBI Synonym Full Names:	centromere protein U
NCBI Official Symbol:	CENPU
NCBI Official Synonym Symbols:	KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
NCBI Protein Information:	centromere protein U
UniProt Protein Name:	Centromere protein U
UniProt Synonym Protein Names:	Centromere protein of 50 kDa; CENP-50; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1
Protein Family:	Centromere protein
UniProt Gene Name:	CENPU
UniProt Entry Name:	CENPU_HUMAN