Name: CDC42BPB Antibody (PACO57052)
SKU: PACO57052
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CDC42BPB Antibody (PACO57052)

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SKU:

PACO57052

Product Type:

Antibody

Reactivity:

Human

Host Species:

Rabbit

Isotype:

IgG

Applications:

ELISA

Applications:

IHC

Applications:

Antibody Type:

Polyclonal Antibody

Conjugation:

Unconjugated

Description
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Description

DatasheetMSDS

Background:

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.

Synonyms:

Serine/threonine-protein kinase MRCK β (EC 2.7.11.1) (CDC42-binding protein kinase beta) (CDC42BP-beta) (DMPK-like beta) (Myotonic dystrophy kinase-related CDC42-binding kinase beta) (MRCK beta) (Myotonic dystrophy protein kinase-like beta), CDC42BPB, KIAA1124

UniProt Protein Function:	MRCKB: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; GEK subfamilyChromosomal Location of Human Ortholog: 14q32.3Cellular Component: actomyosin; cytoplasm; cytoskeleton; intercellular junction; leading edge; plasma membraneMolecular Function: ATP binding; magnesium ion binding; protein kinase activity; protein serine/threonine kinase activity; Rho GTPase bindingBiological Process: actin cytoskeleton reorganization; actomyosin structure organization and biogenesis; cell migration; cytoskeleton organization and biogenesis; establishment and/or maintenance of cell polarity; protein amino acid phosphorylation; signal transduction
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y5S2
NCBI GenInfo Identifier:	92090617
NCBI Gene ID:	9578
NCBI Accession:	Q9Y5S2.2
UniProt Secondary Accession:	Q9Y5S2,Q2L7A5, Q86TJ1, Q9ULU5, A9JR72
UniProt Related Accession:	Q9Y5S2
Molecular Weight:	194,315 Da
NCBI Full Name:	Serine/threonine-protein kinase MRCK beta
NCBI Synonym Full Names:	CDC42 binding protein kinase beta
NCBI Official Symbol:	CDC42BPB
NCBI Official Synonym Symbols:	MRCKB
NCBI Protein Information:	serine/threonine-protein kinase MRCK beta
UniProt Protein Name:	Serine/threonine-protein kinase MRCK beta
UniProt Synonym Protein Names:	CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta; Myotonic dystrophy protein kinase-like beta
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	CDC42BPB
UniProt Entry Name:	MRCKB_HUMAN

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Antibody Name:	CDC42BPB Antibody (PACO57052)
Antibody SKU:	PACO57052
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Serine/threonine-protein kinase MRCK β protein (1609-1711AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Neuroimmunologie: Das Immunsystem des ZNS

Interleukin-8-Signalisierung

Waardenburg-Syndrom und Klein-Waardenburg-Syndrom

CDC42BPB Antibody (PACO57052)

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	IHC image of PACO57052 diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of A549 cells with PACO57052 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO57052 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.