CD3E & CD3G Heterodimer Recombinant Protein (RPES2797)

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SKU:
RPES2797
Product Type:
Recombinant Protein
Species:
Human

Description

system_update_altDatasheet
Product Name:CD3E & CD3G Heterodimer Recombinant Protein (RPES2797)
Product Code:RPES2797
Size:20µg
Species:Human
Expressed Host:HEK293 Cells
Synonyms:CD3E, CD3G Heterodimer,IMD18,T3E,TCRE
Accession:NP_000724.1&NP_000064.1
Sequence:Met 1-Asp126&Met1-Ser116
Fusion tag:
Activity:Measured by its ability to bind OKT3-mIgG2a in a functional ELISA.
Endotoxin:<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:77.2 (40.1+37.1) kDa
AP Mol Mass:42-47 kDa
Formulation:Lyophilized from sterile PBS, pH 7.4.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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