CD3D & CD3E Heterodimer Recombinant Protein (RPES2817)

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SKU:
RPES2817
Product Type:
Recombinant Protein
Species:
Human

Description

system_update_altDatasheet
Product Name:CD3D & CD3E Heterodimer Recombinant Protein (RPES2817)
Product Code:RPES2817
Size:20µg
Species:Human
Expressed Host:HEK293 Cells
Synonyms:T-Cell Surface Glycoprotein CD3 Delta Chain, T-Cell Receptor T3 Delta Chain, CD3d, CD3D, T3D
Accession:P04234&P07766
Sequence:Met 1-Ala105&Met 1-Asp126
Fusion tag:
Activity:Measured by its binding ability in a functional ELISA.Immobilized human CD3D & CD3E Heterodimer Protein at 10 µg/ml (100 ?l/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 µg/ml.
Endotoxin:<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus; constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus; constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
Purity:> 90 % as determined by reducing SDS-PAGE.
Mol Mass:77.5 (37.5+40? kDa
AP Mol Mass:45-50 kDa
Formulation:Lyophilized from sterile PBS; pH 7.4
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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