The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Product Name:
Carbonic Anhydrase 8/CA8 Recombinant Protein (RPES2903)
Measured by its esterase activity. The specific activity is >100 pmoles/min/µg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human CA8 (NP_004047.3) (Met 1-Gln 290) was expressed, with a polyhistidine tag at the C-terminus.
Purity:
> 94 % as determined by reducing SDS-PAGE.
Mol Mass:
33.8 kDa
AP Mol Mass:
37 kDa
Formulation:
Lyophilized from sterile PBS, 15% glycerol, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
