Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.
Product Name:
Carbonic Anhydrase 1/CA1 Recombinant Protein (RPES4109)
Product Code:
RPES4109
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Carbonic Anhydrase 1, Carbonate Dehydratase I, Carbonic Anhydrase B, CAB, Carbonic Anhydrase I, CA-I, CA1
Accession:
P00915
Sequence:
Ala2-Phe261
Fusion tag:
C-6His
Activity:
Measured by its esterase activity. The specific activity is 78.1 pmol/min/µg .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Carbonic Anhydrase 1 is produced by our E.coli expression system and the target gene encoding Ala2-Phe261 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
29.9 kDa
AP Mol Mass:
30 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
system_update_altDatasheetCarbonic Anhydrase 10/CA10 Recombinant Protein Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zin