Antibody Name:	Capsid protein VP1 Antibody (PACO34862)
Antibody SKU:	PACO34862
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA
Recommended Dilutions:
Species Reactivity:	Human parvovirus B19
Immunogen:	Recombinant Human parvovirus B19 Capsid protein VP2 protein (228-781AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Background:

Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids (By similarity).

Synonyms:

Capsid protein VP1 (Coat protein VP1) [Cleaved into: Capsid protein VP2 (Coat protein VP2)]

UniProt Protein Function:	Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids ().
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P07299
NCBI GenInfo Identifier:	116774
NCBI Gene ID:
NCBI Accession:	P07299.1
UniProt Secondary Accession:	P07299
UniProt Related Accession:	P07299
Molecular Weight:
NCBI Full Name:	Capsid protein VP1
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:	Capsid protein VP1
UniProt Synonym Protein Names:	Coat protein VP1
Protein Family:	Capsid protein
UniProt Gene Name:
UniProt Entry Name:	CAPSD_PAVHU