Food Science
CAP (Chloramphenicol) ELISA Kit (FSES0031)
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- SKU:
- FSES0031
- Product Type:
- ELISA Kit
- Applications:
- ELISA
- Research Area:
- Food Science
Description
CAP (Chloramphenicol) ELISA Kit
| Kit Name: | CAP (Chloramphenicol) ELISA Kit | ||||||||||||||||||||||||||
| Kit Size: | 96 Assays | ||||||||||||||||||||||||||
| Test Principle: | This kit uses Competitive-ELISA as the method. It can detect Chloramphenicol (CAP) in samples, such as, muscle, milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, CAP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CAP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of CAP. The concentration of CAP in the samples can be calculated by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||
| Sensitivity: | 0.025 ppb (ng/mL) | ||||||||||||||||||||||||||
| Reaction Mode: | 25°C, 30 min~ 30 min~15 min. | ||||||||||||||||||||||||||
| Detection Limit: | Muscle, Liver--- 0.0125 ppb; Honey, Water ---0.05 ppb;; Eggs---0.1 ppb Urine, Serum, Feed---0.025 ppb; Milk, Milk powder --- 0.075 ppb | ||||||||||||||||||||||||||
| Cross Reactivity: | Chloramphenicol---100%, Thiamphenicol, Florfenicol <0.1%. | ||||||||||||||||||||||||||
| Sample Recovery Rate: | Muscle, Liver--- 85%±20%; Honey --- 85%±25%; Water ---90%±20%; Milk, Feed, Milk powder, Eggs ---75%±25%; Urine, Serum--70%±20%. | ||||||||||||||||||||||||||
| Kits Components: |
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| Instruments: | Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g). | ||||||||||||||||||||||||||
| Micropipette: | Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL). | ||||||||||||||||||||||||||
| Reagents: | Ethyl cetate, N-hexane, Acetonitrile, CH3COONa, Acetic acid, Na2Fe(CN)5(NO)·2H2O, Beta-glucuronidase (activity≥1,000,000 units/g), ZnSO4·7H2O. | ||||||||||||||||||||||||||
| Storage And Expiry Date: | Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box. |
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