Food Science

CAP (Chloramphenicol) ELISA Kit (FSES0031)

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SKU:
FSES0031
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science

Description

Technical Manual

CAP (Chloramphenicol) ELISA Kit

Kit Name: CAP (Chloramphenicol) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Chloramphenicol (CAP) in samples, such as, muscle, milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, CAP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CAP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of CAP. The concentration of CAP in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.025 ppb (ng/mL)
Reaction Mode: 25°C, 30 min~ 30 min~15 min.
Detection Limit: Muscle, Liver--- 0.0125 ppb; Honey, Water ---0.05 ppb;; Eggs---0.1 ppb Urine, Serum, Feed---0.025 ppb; Milk, Milk powder --- 0.075 ppb
Cross Reactivity: Chloramphenicol---100%, Thiamphenicol, Florfenicol <0.1%.
Sample Recovery Rate: Muscle, Liver--- 85%±20%; Honey --- 85%±25%; Water ---90%±20%; Milk, Feed, Milk powder, Eggs ---75%±25%; Urine, Serum--70%±20%.
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1mL each (0 ppb, 0.025 ppb, 0.075 ppb, 0.225 ppb, 0.675 ppb, 2.025 ppb)
HRP Conjugate 11 mL
Antibody Working Solution 5.5 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20 x Wash Buffer 40 mL
2 x Reconstitution Buffer 50 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Ethyl cetate, N-hexane, Acetonitrile, CH3COONa, Acetic acid, Na2Fe(CN)5(NO)·2H2O, Beta-glucuronidase (activity≥1,000,000 units/g), ZnSO4·7H2O.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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