Description
Canine VE-Cadherin ELISA Kit (CNFI00074)
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Canine VE-Cadherin ELISA Kit |
| Product Code: | CNFI00074 |
| Size: | 96 Assays |
| Alias: | Vascular Endothelial Cadherin, Cadherin-5, CD144, CDH, 7B4 |
| Uniprot: | |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | VE-Cadherin ELISA Kit allows for the in vitro quantitative determination of VE-Cadherin concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Sensitivity: | <0.281ng/ml |
| Range: | 0.469-30ng/ml |
| Storage: | 2-8°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%): | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |