Canine VE-Cadherin ELISA Kit (CNFI00074)

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SKU:
CNFI00074
Product Type:
ELISA Kit
Size:
96 Assays
Reactivity:
Canine
Sensitivity:
<0.281ng/ml
Range:
0.469-30ng/ml

Description

Canine VE-Cadherin ELISA Kit (CNFI00074)

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Canine VE-Cadherin ELISA Kit

Product Code:

CNFI00074

Size:

96 Assays

Alias:

Vascular Endothelial Cadherin, Cadherin-5, CD144, CDH, 7B4

Uniprot:

Detection Method:

Sandwich ELISA, Double Antibody

Application:

VE-Cadherin ELISA Kit allows for the in vitro quantitative determination of VE-Cadherin concentrations in serum, plasma, tissue homogenates and other biological fluids.

Sensitivity:

<0.281ng/ml

Range:

0.469-30ng/ml

Storage:

2-8°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.

98

Matrix

Recovery Range (%)

Average (%)

serum(n=5)

88-97

92

EDTA plasma(n=5)

94-103

98

heparin plasma(n=5)

95-103

99

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-97%

87-102%

89-102%

EDTA plasma(n=5)

83-92%

91-101%

86-100%

heparin plasma(n=5)

81-97%

80-96%

82-97%

CV(%):

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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