Description
Product Name: | Canine IFNG Recombinant Protein |
Product Code: | RPPB0404 |
Size: | 20µg |
Species: | Canine |
Target: | IFNG |
Synonyms: | Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation: | The IFNG was lyophilized from a 0.2�m filtered concentrated solution in 25mM Sodium Succinate, pH 5.0, 60mM NaCl, with 0.1% Tween-80. |
Solubility: | It is recommended to reconstitute the lyophilized IFNG in sterile distilled H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
Stability: | Lyophilized IFNG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNG should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Amino Acid Sequence: | QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK |
Biological Activity: | The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV) is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 � 105 IU/mg. |
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Recombinant IFNG Dog produced in E.coli cells is a non-glycosylated, homodimeric protein containing 143 amino acid chain and having a molecular mass of 16.9kDa. IFNG is purified by proprietary chromatographic techniques.
UniProt Protein Function: | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
UniProt Code: | P42161 |
NCBI GenInfo Identifier: | 12644013 |
NCBI Gene ID: | 403801 |
NCBI Accession: | P42161.2 |
UniProt Secondary Accession: | P42161,Q9TVA0, A1E294, |
UniProt Related Accession: | P42161 |
Molecular Weight: | 19,395 Da |
NCBI Full Name: | Interferon gamma |
NCBI Synonym Full Names: | interferon gamma |
NCBI Official Symbol: | IFNG�� |
NCBI Official Synonym Symbols: | IFN-G; IFN-gamma�� |
NCBI Protein Information: | interferon gamma |
UniProt Protein Name: | Interferon gamma |
Protein Family: | Interferon |
UniProt Gene Name: | IFNG�� |