Canine HS (Heparan Sulfate) ELISA Kit (CNFI00073)
- SKU:
- CNFI00073
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Reactivity:
- Canine
- Sensitivity:
- <18.75ng/ml
- Range:
- 31.25-2000ng/ml
Description
Canine HS (Heparan Sulfate) ELISA Kit (CNFI00073)
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Canine HS (Heparan Sulfate) ELISA Kit |
| Product Code: | CNFI00073 |
| Size: | 96 Assays |
| Detection Method: | Competitive ELISA, Coated with Antigen |
| Application: | HS ELISA Kit allows for the in vitro quantitative determination of Heparan Sulfate concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Sensitivity: | <18.75ng/ml |
| Range: | 31.25-2000ng/ml |
| Storage: | 2-8°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Heparan Sulfate and the recovery rates were calculated by comparing the measured value to the expected amount of Heparan Sulfate in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparan Sulfate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%): | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
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HS(Heparan Sulfate) ELISA Kit
