Canine Cytokine Array (40 targets) (SARB0008)

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SKU:
SARB0008

Description

system_update_altDatasheet
Product Sku:SARB0008
Size:2, 4, or 8
Species Detected:Canine
Number of Targets Detected:40
Gene Symbols:C1QTNF1, CCL2, CCL4, CSF2, CXCL1, CXCL8, DCN, EGFR, EPOR, FGF7|HGF, IFNG, IL10, IL12B, IL17A, IL1B, IL2, IL6, KITLG, LGALS3, MET, PDGFB, TNFRSF1A, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Cystatin C
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
Decorin
  • Gene Symbol: DCN DCN1C
  • Gene ID: 403904
  • Accession #: Q29393
EGFR
  • Gene Symbol: EGFR
  • Gene ID: N/A
  • Accession #: Q6QT04
ErbB3
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
Erythropoietin
  • Gene Symbol: EPOR
  • Gene ID: 484943
  • Accession #: Q2KL21
FGF-7 (KGF)
  • Gene Symbol: FGF7 EGK_17479
  • Gene ID: 574345
  • Accession #: F7A1B0
Galectin-3
  • Gene Symbol: LGALS3
  • Gene ID:
  • Accession #: P38486
GASP-1 (WFIKKNRP)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 403923
  • Accession #: P48749
GRO alpha (CXCL1)
  • Gene Symbol: Cxcl1
  • Gene ID: N/A
  • Accession #: N/A
HGF
  • Gene Symbol: HGF
  • Gene ID: 403441
  • Accession #: Q867B7
HGFR
  • Gene Symbol: MET
  • Gene ID: 403438
  • Accession #: Q75ZY9
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 403801
  • Accession #: P42161
IGFBP-2
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID:
  • Accession #: Q28292
IL-1 Ra (IL-1 F3) IL-10
  • Gene Symbol: IL10
  • Gene ID: 403628
  • Accession #: P48411
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 403976
  • Accession #: Q28268
IL-13
IL-17A
  • Gene Symbol: IL-17A IL17A
  • Gene ID: 481837
  • Accession #: C6L8D7
IL-2
  • Gene Symbol: IL2
  • Gene ID: 403989
  • Accession #: Q29416
IL-21 IL-6
  • Gene Symbol: IL6
  • Gene ID: 403985
  • Accession #: P41323
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 403850
  • Accession #: P41324
MCP-1 (CCL2)
  • Gene Symbol: CCL2 MCP1 SCYA2
  • Gene ID: 403981
  • Accession #: P52203
MIP-1 beta (CCL4)
  • Gene Symbol: CCL4 SCYA4
  • Gene ID: 448786
  • Accession #: Q68AZ0
MMP-8
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
NCAM-1 (CD56)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
Nope
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 442986
  • Accession #: Q6Q7I7
RAGE
  • Gene Symbol: N/A
  • Gene ID: 403168
  • Accession #: Q24LN3
RANTES (CCL5) Resistin
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
SCF
  • Gene Symbol: KITLG MGF
  • Gene ID: 403507
  • Accession #: Q06220
TGF alpha
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 403922
  • Accession #: P51742
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 403634;
  • Accession #: F1PNY7
Trappin-2
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 403802
  • Accession #: Q9MYV3
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Canine Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Cystatin C
Analytes:
Decorin
Analytes:
EGFR
Analytes:
ErbB3
Analytes:
Erythropoietin
Analytes:
FGF-7 (KGF)
Analytes:
Galectin-3
Analytes:
GASP-1 (WFIKKNRP)
Analytes:
GM-CSF
Analytes:
GRO alpha (CXCL1)
Analytes:
HGF
Analytes:
HGFR
Analytes:
IFN-gamma
Analytes:
IGFBP-2
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-1 Ra (IL-1 F3)
Analytes:
IL-10
Analytes:
IL-12 p40
Analytes:
IL-13
Analytes:
IL-17A
Analytes:
IL-2
Analytes:
IL-21
Analytes:
IL-6
Analytes:
IL-8 (CXCL8)
Analytes:
MCP-1 (CCL2)
Analytes:
MIP-1 beta (CCL4)
Analytes:
MMP-8
Analytes:
NCAM-1 (CD56)
Analytes:
Nope
Analytes:
PDGF-BB
Analytes:
RAGE
Analytes:
RANTES (CCL5)
Analytes:
Resistin
Analytes:
SCF
Analytes:
TGF alpha
Analytes:
TNF alpha
Analytes:
TNF RI (TNFRSF1A)
Analytes:
Trappin-2
Analytes:
VEGF-A
Reactivity:
Canine
Applications:
Multiplex Array
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