Ca2+-ATPase Activity Assay Kit
- SKU:
- MAES0213
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Animal Tissue
- Sensitivity:
- 1.18 U/kg wet weight
- Range:
- 1.18-286.43 U/kg
- Assay Time:
- 90 min
Description
| Product Name: | Ca2+-ATPase Activity Assay Kit |
| Product Code: | MAES0213 |
| Sample Type: | Animal Tissue |
| Assay Time: | 90 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | Ca2+-ATP |
| Detection Instrument: | Microplate reader (660 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 1.18 U/kg wet weight |
| Detection Range: | 1.18-286.43 U/kg wet weight |
| Inter-assay CV: | 0.08 |
| Intra-assay CV: | 0.06 |
ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+ -ATPase activity was inhibited, while in the sample system, Ca2+ -ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+ -ATPase during the incubation time. The activity of Ca2+ -ATPase was determined by inorganic phosphorus production.
| Storage: | 2-8°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Ultrapure water, Normal saline (0.9% NaCl) |
| Other Instruments Required: | Test tube, Vortex mixer, Incubator, Centrifuge, 100°C water bath, Microplate reader |
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