BUD13 Antibody (PACO61157)
- SKU:
- PACO61157
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | BUD13 Antibody (PACO61157) |
| Antibody SKU: | PACO61157 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human BUD13 homolog protein (132-233AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61157 diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of U251 cells with PACO61157 at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | nucleus, RES complex, RNA binding, mRNA export from nucleus, mRNA splicing, via spliceosome |
| Synonyms: | BUD13 homolog, BUD13 |
| UniProt Protein Function: | BUD13: a conserved protein of unknown function.Protein type: RNA splicing; SpliceosomeChromosomal Location of Human Ortholog: 11q23.3Cellular Component: nucleusMolecular Function: protein bindingBiological Process: mRNA export from nucleus; nuclear mRNA splicing, via spliceosome |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9BRD0 |
| NCBI GenInfo Identifier: | 74732865 |
| NCBI Gene ID: | 84811 |
| NCBI Accession: | Q9BRD0.1 |
| UniProt Secondary Accession: | Q9BRD0,Q96LS7, A8K0S0 |
| UniProt Related Accession: | Q9BRD0 |
| Molecular Weight: | 55,567 Da |
| NCBI Full Name: | BUD13 homolog |
| NCBI Synonym Full Names: | BUD13 homolog |
| NCBI Official Symbol: | BUD13 |
| NCBI Official Synonym Symbols: | Cwc26; fSAP71 |
| NCBI Protein Information: | BUD13 homolog |
| UniProt Protein Name: | BUD13 homolog |
| UniProt Synonym Protein Names: | |
| Protein Family: | BUD13 |
| UniProt Gene Name: | BUD13 |
| UniProt Entry Name: | BUD13_HUMAN |
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