BROX Antibody (PACO61005)
- SKU:
- PACO61005
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | BROX Antibody (PACO61005) |
| Antibody SKU: | PACO61005 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human BRO1 domain-containing protein BROX protein (288-409AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61005 diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO61005 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Western Blot. Positive WB detected in: K562 whole cell lysate. All lanes: BROX antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 43 kDa. Observed band size: 47 kDa. |
| Background: | extracellular exosome |
| Synonyms: | BRO1 domain-containing protein BROX (BRO1 domain- and CAAX motif-containing protein), BROX, BROFTI C1orf58 |
| UniProt Protein Function: | BROX: Belongs to the BROX family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 1q41Cellular Component: membrane |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q5VW32 |
| NCBI GenInfo Identifier: | 568384731 |
| NCBI Gene ID: | 148362 |
| NCBI Accession: | NP_001275508.1 |
| UniProt Secondary Accession: | Q5VW32,Q96MG1, B7Z9G5 |
| UniProt Related Accession: | Q5VW32 |
| Molecular Weight: | 42,872 Da |
| NCBI Full Name: | BRO1 domain-containing protein BROX isoform b |
| NCBI Synonym Full Names: | BRO1 domain and CAAX motif containing |
| NCBI Official Symbol: | BROX |
| NCBI Official Synonym Symbols: | C1orf58 |
| NCBI Protein Information: | BRO1 domain-containing protein BROX |
| UniProt Protein Name: | BRO1 domain-containing protein BROX |
| UniProt Synonym Protein Names: | BRO1 domain- and CAAX motif-containing protein |
| Protein Family: | BRO1 domain-containing protein |
| UniProt Gene Name: | BROX |
| UniProt Entry Name: | BROX_HUMAN |
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