BRAF V600E Monoclonal Antibody
- SKU:
- CAB24020
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- Applications:
- IF
Description
| Product Name: | BRAF V600E Monoclonal Antibody |
| Product Code: | CAB24020 |
| Reactivity: | Human |
| Applications: | Western blotting, Immunofluorescence |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC62593 |
| Reactivity: | Human |
| Tested Applications: | WB, IF/ICC, ELISA |
| Key Applications: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:1000, -1:2000, IF 1:50,-1 : 200 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | 293F, 293F-BRAF V600E-Avi-His-N, 293F-BRAF-Avi-His-N |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 507-606 of B-Raf (NP_004324.2). |
| Sequence: | LRKTR HVNIL LFMGY STKPQ LAIVT QWCEG SSLYH HLHII ETKFE MIKLI DIARQ TAQGM DYLHA KSIIH RDLKS NNIFL HEDLT VKIGD FGLAT VKSRW SG |
| Synonyms: | NS7, B-raf, BRAF1, RAFB1, B-RAF1, BRAF-1 |
| Calculated MW: | 84kDa |
| Observed MW: | 85kDa |
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