BOLA2 Antibody (PACO60344)
- SKU:
- PACO60344
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | BOLA2 Antibody (PACO60344) |
| Antibody SKU: | PACO60344 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human BolA-like protein 2 protein (1-86AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60344 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO60344 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60344 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins. Acts together with the monothiol glutaredoxin GLRX3. |
| Synonyms: | BolA-like protein 2, BOLA2; BOLA2B, BOLA2A; |
| UniProt Protein Function: | BOLA2B: Belongs to the bolA/yrbA family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16p11.2 |
| UniProt Protein Details: | |
| NCBI Summary: | This gene is located within a region of a segmental duplication on chromosome 16p. The product of this gene belongs to the eukaryotic subfamily of the BolA-like proteins. This gene encodes the BolA-like protein 2. The BolA-like proteins are widely conserved from prokaryotes to eukaryotes, and these proteins seem to be involved in cell proliferation or cell-cycle regulation. This duplicated region contains a complete copy of the short splice variant of the BOLA2 gene and a partial copy of the long splice variant of the BOLA2 gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H3K6 |
| NCBI GenInfo Identifier: | 85797673 |
| NCBI Gene ID: | 654483 |
| NCBI Accession: | NP_001034271.1 |
| UniProt Secondary Accession: | Q9H3K6,A1L454, A7YDY3 |
| UniProt Related Accession: | Q9H3K6 |
| Molecular Weight: | 6,842 Da |
| NCBI Full Name: | bolA-like protein 2 |
| NCBI Synonym Full Names: | bolA family member 2B |
| NCBI Official Symbol: | BOLA2B |
| NCBI Official Synonym Symbols: | BOLA2 |
| NCBI Protein Information: | bolA-like protein 2; bolA-like 2B; bolA homolog 2B; bolA-like protein 2B; BolA-like protein 2 member B |
| UniProt Protein Name: | BolA-like protein 2 |
| UniProt Synonym Protein Names: | |
| Protein Family: | BolA-like protein |
| UniProt Gene Name: | BOLA2 |
| UniProt Entry Name: | BOLA2_HUMAN |
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