Antibody Name:	Birc5 Antibody (PACO25264)
Antibody SKU:	PACO25264
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:	Mouse, Human
Immunogen:	Recombinant Mouse Baculoviral IAP repeat-containing protein 5 protein (1-140AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate
All lanes: Birc5 antibody at 5.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17, 15, 5 kDa
Observed band size: 17 kDa
.

Background:

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7.

Synonyms:

Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor 4) (Apoptosis inhibitor survivin) (TIAP), Birc5, Api4 Iap4

UniProt Protein Function:	Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.
UniProt Protein Details:	Protein type:Apoptosis Cellular Component: apical plasma membrane; centriole; chromosome; chromosome, pericentric region; cytoplasm; cytoplasmic microtubule; cytoskeleton; cytosol; interphase microtubule organizing center; kinetochore; microtubule; midbody; nuclear chromosome; nucleus; spindle microtubule Molecular Function:caspase inhibitor activity; chaperone binding; cofactor binding; cysteine protease inhibitor activity; enzyme binding; identical protein binding; metal ion binding; microtubule binding; protease inhibitor activity; protein binding; protein heterodimerization activity; protein homodimerization activity; Ran GTPase binding; tubulin binding; ubiquitin-protein ligase activity; zinc ion binding Biological Process: apoptosis; cell cycle; cell division; chromosome segregation; cytokinesis; embryonic development; establishment of chromosome localization; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; mitosis; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of neuron apoptosis; negative regulation of peptidase activity; negative regulation of transcription, DNA-dependent; positive regulation of cell cycle; positive regulation of exit from mitosis; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; protein complex localization; regulation of mitotic cell cycle; regulation of signal transduction; regulation of transcription, DNA-dependent; spindle checkpoint; transcription, DNA-dependent
NCBI Summary:	This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. In humans, gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts have been identified in human that regulate this gene's expression. At least three transcript variants encoding distinct isoforms have been found for this gene, although at least one of these transcript variants is a nonsense-mediated decay (NMD) candidate. [provided by RefSeq, Jul 2008]
UniProt Code:	O70201
NCBI GenInfo Identifier:	59859890
NCBI Gene ID:	11799
NCBI Accession:	NP_001012273.1
UniProt Related Accession:	O70201
Molecular Weight:	16kDa
NCBI Full Name:	baculoviral IAP repeat-containing protein 5 isoform 3
NCBI Synonym Full Names:	baculoviral IAP repeat-containing 5
NCBI Official Symbol:	Birc5
NCBI Official Synonym Symbols:	Api4; TIAP; AAC-11; survivin40
NCBI Protein Information:	baculoviral IAP repeat-containing protein 5
UniProt Protein Name:	Baculoviral IAP repeat-containing protein 5
UniProt Synonym Protein Names:	Apoptosis inhibitor 4; Apoptosis inhibitor survivin; TIAP
UniProt Gene Name:	Birc5
UniProt Entry Name:	BIRC5_MOUSE