Bile Acid Assay Kit (BA0099)

Bile Acid Assay Kit

BILE ACIDS.Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist in a sulfate salt form known as bile acid-sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. This assay does not measure bile acid-sulfates, and measures only the twelve non-sulfated bile acids.

Assay Genie's Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3-alpha-hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (Ex/Em = 530/585 nm) is linear to the bile acid concentration in the sample.

Applications

For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. Linear detection range of 1 - 150 uM bile acids.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Bile Acid Assay Kit Information:

Kit Includes:	Assay Buffer: 10 mL Enzyme A: 120 uL NAD Solution: 1 mL Enzyme B: 120 uL Probe: 750 uL Standard: 120 uL
Kit Requires:	Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and fluorescent plate reader capable of reading λexc/em = 530/585 nm
Method of Detection:	FL530/585nm
Detection Limit:	1 uM
Samples:	Serum, plasma, urine and other biological samples.
Species:	All
Protocol Length:	20 min
Size:	100 tests
Storage:	Store all reagents at -20°C
Shelf Life:	6 months