Beta-Glucosidase Activity Assay Kit (BA0018)

beta-Glucosidase Activity Assay Kit - Information

Assay Genie's beta-Glucosidase Assay Kit is designed to measure beta-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-beta-D-glucopyranoside that is hydrolyzed specifically by beta-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Applications

For quantitative determination of beta-glucosidase activity and evaluation of drug effects on its metabolism.

beta-Glucosidase Activity Assay Kit - Key Features

• High sensitivity and wide linear range. Use 20 sample. The detection limit is 2 U/L, linear up to 250 U/L.
• Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of beta-glucosidase activity within 20 minutes.
• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

beta-Glucosidase Activity Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 24 mL (pH 7.0) b-NPG Substrate: 1 mL Calibrator: 10 mL (equivalent to 250 U/L)
Kit Requires	Pipeting devices and accessories (e.g. multi-channel pipettor). Clear bottom 96-well plates and plate reader.
Method of Detection	OD405nm
Detection Limit	2 U/L
Samples	Biological
Species	All
Protocol Length	20 min
Size	100 tests
Storage	Store all components at -20°C.
Shelf Life	6 months

More Details

beta-GLUCOSIDASE is a glucosidase enzyme which acts upon beta1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). beta-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a beta-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a beta-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher's disease and Parkinson's disease. Simple, direct and automation-ready procedures for measuring beta-glucosidase activity are becoming popular in Research and Drug Discovery.