BAZ2B Antibody (PACO57536)
- SKU:
- PACO57536
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | BAZ2B Antibody (PACO57536) |
| Antibody SKU: | PACO57536 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Bromodomain adjacent to zinc finger domain protein 2B protein (139-240AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of Hela cells with PACO57536 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57536 diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO57536 diluted at 1:800 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | May play a role in transcriptional regulation interacting with ISWI. |
| Synonyms: | Bromodomain adjacent to zinc finger domain protein 2B (hWALp4), BAZ2B, KIAA1476 |
| UniProt Protein Function: | BAZ2B: May play a role in transcriptional regulation interacting with ISWI. Belongs to the WAL family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription regulationChromosomal Location of Human Ortholog: 2q24.2Cellular Component: nucleusMolecular Function: protein binding; DNA binding; zinc ion bindingBiological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent |
| UniProt Protein Details: | |
| NCBI Summary: | This gene belongs to the bromodomain gene family. Members of this gene family encode proteins that are integral components of chromatin remodeling complexes. The encoded protein showed strong preference for the activating H3K14Ac mark in a histone peptide screen, suggesting a potential role in transcriptional activation. This gene may be associated with susceptibility to sudden cardiac death (SCD). [provided by RefSeq, Aug 2016] |
| UniProt Code: | Q9UIF8 |
| NCBI GenInfo Identifier: | 586798142 |
| NCBI Gene ID: | 29994 |
| NCBI Accession: | NP_001276904.1 |
| UniProt Secondary Accession: | Q9UIF8 |
| UniProt Related Accession: | Q9UIF8 |
| Molecular Weight: | 48kDa |
| NCBI Full Name: | bromodomain adjacent to zinc finger domain protein 2B isoform b |
| NCBI Synonym Full Names: | bromodomain adjacent to zinc finger domain 2B |
| NCBI Official Symbol: | BAZ2B |
| NCBI Official Synonym Symbols: | WALp4 |
| NCBI Protein Information: | bromodomain adjacent to zinc finger domain protein 2B |
| UniProt Protein Name: | Bromodomain adjacent to zinc finger domain protein 2B |
| UniProt Synonym Protein Names: | hWALp4 |
| Protein Family: | Bromodomain adjacent to zinc finger domain protein |
| UniProt Gene Name: | BAZ2B |
| UniProt Entry Name: | BAZ2B_HUMAN |
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