Description
Antibody Name: | ATP6V0D2 Antibody (PACO62527) |
Antibody SKU: | PACO62527 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate. All lanes: ATP6V0D2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO62527 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO62527 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acid, fying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). |
Synonyms: | V-type proton ATPase subunit d 2 (V-ATPase subunit d 2) (Vacuolar proton pump subunit d 2), ATP6V0D2 |
UniProt Protein Function: | |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | Q8N8Y2 |
NCBI GenInfo Identifier: | 22749165 |
NCBI Gene ID: | 245972 |
NCBI Accession: | NP_689778.1 |
UniProt Secondary Accession: | Q8N8Y2 |
UniProt Related Accession: | Q8N8Y2 |
Molecular Weight: | |
NCBI Full Name: | V-type proton ATPase subunit d 2 |
NCBI Synonym Full Names: | ATPase H+ transporting V0 subunit d2 |
NCBI Official Symbol: | ATP6V0D2 |
NCBI Official Synonym Symbols: | VMA6; ATP6D2 |
NCBI Protein Information: | V-type proton ATPase subunit d 2 |
UniProt Protein Name: | V-type proton ATPase subunit d 2 |
UniProt Synonym Protein Names: | Vacuolar proton pump subunit d 2 |
Protein Family: | |
UniProt Gene Name: | ATP6V0D2 |
UniProt Entry Name: | VA0D2_HUMAN |