ATP6V0D2 Antibody (PACO62527)
- SKU:
- PACO62527
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ATP6V0D2 Antibody (PACO62527) |
| Antibody SKU: | PACO62527 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate. All lanes: ATP6V0D2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO62527 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO62527 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acid, fying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). |
| Synonyms: | V-type proton ATPase subunit d 2 (V-ATPase subunit d 2) (Vacuolar proton pump subunit d 2), ATP6V0D2 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8N8Y2 |
| NCBI GenInfo Identifier: | 22749165 |
| NCBI Gene ID: | 245972 |
| NCBI Accession: | NP_689778.1 |
| UniProt Secondary Accession: | Q8N8Y2 |
| UniProt Related Accession: | Q8N8Y2 |
| Molecular Weight: | |
| NCBI Full Name: | V-type proton ATPase subunit d 2 |
| NCBI Synonym Full Names: | ATPase H+ transporting V0 subunit d2 |
| NCBI Official Symbol: | ATP6V0D2 |
| NCBI Official Synonym Symbols: | VMA6; ATP6D2 |
| NCBI Protein Information: | V-type proton ATPase subunit d 2 |
| UniProt Protein Name: | V-type proton ATPase subunit d 2 |
| UniProt Synonym Protein Names: | Vacuolar proton pump subunit d 2 |
| Protein Family: | |
| UniProt Gene Name: | ATP6V0D2 |
| UniProt Entry Name: | VA0D2_HUMAN |
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