ATP6AP1L Antibody (PACO63423)
- SKU:
- PACO63423
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ATP6AP1L Antibody (PACO63423) |
| Antibody SKU: | PACO63423 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence from Human V-type proton ATPase subunit S1-like protein (190-209AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63423 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of SH-SY5Y cells with PACO63423 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Synonyms: | V-type proton ATPase subunit S1-like protein (Vacuolar proton pump subunit S1-like protein), ATP6AP1L |
| UniProt Protein Function: | ATP6AP1L: Belongs to the vacuolar ATPase subunit S1 family.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 5q14.2Cellular Component: integral to membraneMolecular Function: hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanismBiological Process: ATP hydrolysis coupled proton transport |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q52LC2 |
| NCBI GenInfo Identifier: | 63003910 |
| NCBI Gene ID: | 92270 |
| NCBI Accession: | NP_001017971.1 |
| UniProt Secondary Accession: | Q52LC2 |
| UniProt Related Accession: | Q52LC2 |
| Molecular Weight: | 25,310 Da |
| NCBI Full Name: | V-type proton ATPase subunit S1-like protein |
| NCBI Synonym Full Names: | ATPase, H+ transporting, lysosomal accessory protein 1-like |
| NCBI Official Symbol: | ATP6AP1L |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | V-type proton ATPase subunit S1-like protein; vacuolar proton pump subunit S1-like protein |
| UniProt Protein Name: | V-type proton ATPase subunit S1-like protein |
| UniProt Synonym Protein Names: | Vacuolar proton pump subunit S1-like protein |
| Protein Family: | V-type proton ATPase subunit S1-like protein |
| UniProt Gene Name: | ATP6AP1L |
| UniProt Entry Name: | VAS1L_HUMAN |
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