ATP2B3 Antibody (PACO62447)
- SKU:
- PACO62447
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ATP2B3 Antibody (PACO62447) |
| Antibody SKU: | PACO62447 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:100-1:1000, IF:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Plasma membrane calcium-transporting ATPase 3 protein (1057-1220AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: ATP2B3 antibody at 1:500. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 135, 129, 128, 133, 127, 125, 123 kDa. Observed band size: 135 kDa. |
 | Immunofluorescence staining of Hela cells with PACO62447 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. |
| Synonyms: | Plasma membrane calcium-transporting ATPase 3 (PMCA3) (EC 3.6.3.8) (Plasma membrane calcium ATPase isoform 3) (Plasma membrane calcium pump isoform 3), ATP2B3 |
| UniProt Protein Function: | ATP2B3: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 8 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Transporter; Hydrolase; Membrane protein, multi-pass; Transporter, ion channel; EC 3.6.3.8Chromosomal Location of Human Ortholog: Xq28Cellular Component: Golgi apparatus; integral to plasma membrane; intracellular membrane-bound organelle; plasma membraneMolecular Function: calcium-transporting ATPase activity; PDZ domain bindingBiological Process: cellular calcium ion homeostasis; transportDisease: Spinocerebellar Ataxia, X-linked 1 |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16720 |
| NCBI GenInfo Identifier: | 116241261 |
| NCBI Gene ID: | 492 |
| NCBI Accession: | Q16720.3 |
| UniProt Secondary Accession: | Q16720,Q12995, Q16858, B7WNR8, B7WNY5 |
| UniProt Related Accession: | Q16720 |
| Molecular Weight: | 122,760 Da |
| NCBI Full Name: | Plasma membrane calcium-transporting ATPase 3 |
| NCBI Synonym Full Names: | ATPase plasma membrane Ca2+ transporting 3 |
| NCBI Official Symbol: | ATP2B3 |
| NCBI Official Synonym Symbols: | CLA2; OPCA; PMCA3; SCAX1; CFAP39; PMCA3a |
| NCBI Protein Information: | plasma membrane calcium-transporting ATPase 3 |
| UniProt Protein Name: | Plasma membrane calcium-transporting ATPase 3 |
| UniProt Synonym Protein Names: | Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium pump isoform 3 |
| Protein Family: | Plasma membrane calcium-transporting ATPase |
| UniProt Gene Name: | ATP2B3 |
| UniProt Entry Name: | AT2B3_HUMAN |
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