Description
Product Name: | Asymmetric DiMethyl-Histone H3-R8 Monoclonal Antibody |
Product Code: | CAB22144 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Applications: | Western blotting, Immunohistochemistry |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Clone No: | ARC54909 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Tested Applications: | WB, IHC-P, ELISA, DB |
Key Applications: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:2000-1:4000, IHC-P 1:50-1:200, DB 1:5000-1:8000 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Positive Samples: | HeLa, NIH/3T3, C6 |
Cellular Location: | Chromosome, nucleus |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Immunogen: | A synthetic asymmetric dimethylated peptide around R8 of human Histone H3 (NP_003520.1). |
Sequence: | MARTK QTARK STGGK APRKQ LATKA ARKSA PATGG VKKPH RYRPG TVALR EIRRY QKSTE LLIRK LPFQR LVREI AQDFK TDLRF QSSAV MALQE ACEAY |
Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
Calculated MW: | 16kDa |
Observed MW: | 17kDa |