Metabolism Assays

Aspartate Assay Kit (BA0078)

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SKU:
BA0078
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Plasma, Serum, Tissue, Culture Media
Research Area:
Urea
Research Area:
Amino Acid and Protein
Research Area:
Clinical Chemistry

Description

ELISA Kit Technical ManualMSDS

Aspartate Assay Kit - Information

Assay Genie's Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the aspartate concentration in the sample.

Applications

For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

Aspartate Assay Kit - Key Features

  • Sensitive and accurate. Linear detection range: 2 to 400 aspartate for colorimetric assays and 1 to 50 for fluorimetric assays.

Aspartate Assay Kit - Data Sheet

Kit IncludesDeveloper: 10 mL AST Enzyme: 240 mL Dye Reagent: 120 mL Cosubstrate: 600 mL ODC Enzyme: 120 mL Aspartate Standard: 400 mL
Kit RequiresPipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.
Method of DetectionOD570nm, or FL530/585nm
Detection LimitOD, FL: 2, 1
SamplesPlasma, serum, tissue, culture media etc
SpeciesAll
Protocol Length60 min
Size100 tests
StorageStore all kit components at -20 °C.
Shelf Life6 months

More Details

ASPARTATE , a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis.
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