ARRDC1 Antibody (PACO59329)
- SKU:
- PACO59329
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ARRDC1 Antibody (PACO59329) |
| Antibody SKU: | PACO59329 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Arrestin domain-containing protein 1 protein (288-433AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59329 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of MCF-7 cells with PACO59329 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59329 diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | cytoplasmic vesicle, extracellular exosome, plasma membrane |
| Synonyms: | Arrestin domain-containing protein 1 (Alpha-arrestin 1), ARRDC1 |
| UniProt Protein Function: | ARRDC1: Belongs to the arrestin family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 9q34.3Cellular Component: cytoplasmic vesicle; plasma membrane |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8N5I2 |
| NCBI GenInfo Identifier: | 74751028 |
| NCBI Gene ID: | 92714 |
| NCBI Accession: | Q8N5I2.1 |
| UniProt Secondary Accession: | Q8N5I2 |
| UniProt Related Accession: | Q8N5I2 |
| Molecular Weight: | 45,981 Da |
| NCBI Full Name: | Arrestin domain-containing protein 1 |
| NCBI Synonym Full Names: | arrestin domain containing 1 |
| NCBI Official Symbol: | ARRDC1 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | arrestin domain-containing protein 1 |
| UniProt Protein Name: | Arrestin domain-containing protein 1 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Arrestin domain-containing protein |
| UniProt Gene Name: | ARRDC1 |
| UniProt Entry Name: | ARRD1_HUMAN |
| Antibodies |
| ARRDC1 Antibody (PACO06265) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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