Name: ARPC4 Antibody (PACO56318)
SKU: PACO56318
Availability: OutOfStock

Antibody Name:	ARPC4 Antibody (PACO56318)
Antibody SKU:	PACO56318
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Actin-related protein 2/3 complex subunit 4 protein (2-168AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO56318 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with PACO56318 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Synonyms:	Actin-related protein 2/3 complex subunit 4 (Arp2/3 complex 20 kDa subunit) (p20-ARC), ARPC4, ARC20

UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:	This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010]
UniProt Code:	P59998
NCBI GenInfo Identifier:	311771642
NCBI Gene ID:	10093
NCBI Accession:	NM_005718.4
UniProt Secondary Accession:	P59998
UniProt Related Accession:	P59998
Molecular Weight:
NCBI Full Name:	Homo sapiens actin related protein 2/3 complex subunit 4 (ARPC4), transcript variant 1, mRNA
NCBI Synonym Full Names:	actin related protein 2/3 complex subunit 4
NCBI Official Symbol:	ARPC4
NCBI Official Synonym Symbols:	ARC20; P20-ARC
NCBI Protein Information:	actin-related protein 2/3 complex subunit 4
UniProt Protein Name:	Actin-related protein 2/3 complex subunit 4
UniProt Synonym Protein Names:	Arp2/3 complex 20 kDa subunit
Protein Family:	Actin-related protein 2/3 complex
UniProt Gene Name:	ARPC4
UniProt Entry Name:	ARPC4_HUMAN