Antibody Name:	ARIH2 Antibody (PACO47306)
Antibody SKU:	PACO47306
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:2000-1:5000, IHC:1:20-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human E3 ubiquitin-protein ligase ARIH2 protein (43-360AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue. All lanes: ARIH2 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa..
	Immunohistochemistry of paraffin-embedded human lymph node tissue using PACO47306 at dilution of 1:100.

Background:

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex. Mediates 'Lys-6', 'Lys-48'-and 'Lys-63'-linked polyubiquitination. May play a role in myelopoiesis.

Synonyms:

E3 ubiquitin-protein ligase ARIH2 (ARI-2) (Protein ariadne-2 homolog) (EC 2.3.2.27) (RING-type E3 ubiquitin transferase ARIH2) (Triad1 protein), ARIH2, ARI2 TRIAD1

UniProt Protein Function:	ARIH2: E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys- 63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis. Belongs to the RBR family. Ariadne subfamily.Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-Chromosomal Location of Human Ortholog: 3p21Cellular Component: cytoplasm; nucleus; ubiquitin ligase complexMolecular Function: protein binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activityBiological Process: developmental cell growth; multicellular organismal development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]
UniProt Code:	O95376
NCBI GenInfo Identifier:	18202259
NCBI Gene ID:	10425
NCBI Accession:	O95376.1
UniProt Secondary Accession:	O95376,Q9HBZ6, Q9UEM9
UniProt Related Accession:	O95376
Molecular Weight:	57,819 Da
NCBI Full Name:	E3 ubiquitin-protein ligase ARIH2
NCBI Synonym Full Names:	ariadne RBR E3 ubiquitin protein ligase 2
NCBI Official Symbol:	ARIH2
NCBI Official Synonym Symbols:	ARI2; TRIAD1
NCBI Protein Information:	E3 ubiquitin-protein ligase ARIH2
UniProt Protein Name:	E3 ubiquitin-protein ligase ARIH2
UniProt Synonym Protein Names:	Triad1 protein
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	ARIH2
UniProt Entry Name:	ARI2_HUMAN