Name: ARHGEF18 Antibody (PACO29504)
SKU: PACO29504
Availability: OutOfStock

Antibody Name:	ARHGEF18 Antibody (PACO29504)
Antibody SKU:	PACO29504
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Rho guanine nucleotide exchange factor 18 protein (159-551AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO29504 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of PC3 cells with PACO29504 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-γ (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950).

Synonyms:

Rho guanine nucleotide exchange factor 18 (114 kDa Rho-specific guanine nucleotide exchange factor) (p114-Rho-GEF) (p114RhoGEF) (Septin-associated RhoGEF) (SA-RhoGEF), ARHGEF18, KIAA0521

UniProt Protein Function:	ARHGEF18: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. 2 isoforms of the human protein are produced by alternative splicing.Protein type: GEFs; Motility/polarity/chemotaxis; GEFs, Rac/RhoChromosomal Location of Human Ortholog: 19p13.3Cellular Component: apical part of cell; cell junction; cytoplasm; cytosolMolecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activityBiological Process: actin cytoskeleton organization and biogenesis; positive regulation of apoptosis; regulation of cell shape; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway
UniProt Protein Details:
NCBI Summary:	Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
UniProt Code:	Q6ZSZ5
NCBI GenInfo Identifier:	296439444
NCBI Gene ID:	23370
NCBI Accession:	Q6ZSZ5.3
UniProt Secondary Accession:	Q6ZSZ5,O60274, Q6DD92, A8MV62, B5ME81
UniProt Related Accession:	Q6ZSZ5
Molecular Weight:	114,077 Da
NCBI Full Name:	Rho guanine nucleotide exchange factor 18
NCBI Synonym Full Names:	Rho/Rac guanine nucleotide exchange factor 18
NCBI Official Symbol:	ARHGEF18
NCBI Official Synonym Symbols:	P114-RhoGEF
NCBI Protein Information:	rho guanine nucleotide exchange factor 18
UniProt Protein Name:	Rho guanine nucleotide exchange factor 18
UniProt Synonym Protein Names:	114 kDa Rho-specific guanine nucleotide exchange factor; p114-Rho-GEF; p114RhoGEF; Septin-associated RhoGEF; SA-RhoGEF
Protein Family:	Rho guanine nucleotide exchange factor
UniProt Gene Name:	ARHGEF18
UniProt Entry Name:	ARHGI_HUMAN