ARHGAP32 Antibody (PACO61622)
- SKU:
- PACO61622
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ARHGAP32 Antibody (PACO61622) |
| Antibody SKU: | PACO61622 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Rho GTPase-activating protein 32 protein (130-205AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of SH-SY5Y cells with PACO61622 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity (By similarity). |
| Synonyms: | Rho GTPase-activating protein 32, Brain-specific Rho GTPase-activating protein, GAB-associated Cdc42/Rac GTPase-activating protein, GC-GAP, GTPase regulator interacting with TrkA, Rho-type GTPase-activating protein 32, Rho/Cdc42/Rac GTPase-activating protein RICS, RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling, p200RhoGAP, p250GAP, ARHGAP32, GRIT, KIAA0712, RICS |
| UniProt Protein Function: | ARHGAP32: GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity. Belongs to the PX domain-containing GAP family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: GAPs; GAPs, Rac/RhoChromosomal Location of Human Ortholog: 11q24.3Cellular Component: Golgi membrane; postsynaptic membrane; endoplasmic reticulum membrane; postsynaptic density; dendritic spine; endosome membrane; cell cortex; cytosol; cell junction; actin cytoskeletonMolecular Function: protein binding; GTPase activator activity; phosphoinositide bindingBiological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity |
| UniProt Protein Details: | |
| NCBI Summary: | RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | A7KAX9 |
| NCBI GenInfo Identifier: | 218083800 |
| NCBI Gene ID: | 9743 |
| NCBI Accession: | NP_001136157.1 |
| UniProt Secondary Accession: | A7KAX9,O94820, Q86YL6, Q8IUG4, Q9BWG3, I7H0B0 |
| UniProt Related Accession: | A7KAX9 |
| Molecular Weight: | 36,669 Da |
| NCBI Full Name: | rho GTPase-activating protein 32 isoform 1 |
| NCBI Synonym Full Names: | Rho GTPase activating protein 32 |
| NCBI Official Symbol: | ARHGAP32 |
| NCBI Official Synonym Symbols: | GRIT; RICS; GC-GAP; PX-RICS; p250GAP; p200RhoGAP |
| NCBI Protein Information: | rho GTPase-activating protein 32 |
| UniProt Protein Name: | Rho GTPase-activating protein 32 |
| UniProt Synonym Protein Names: | Brain-specific Rho GTPase-activating protein; GAB-associated Cdc42/Rac GTPase-activating protein; GC-GAP; GTPase regulator interacting with TrkA; Rho-type GTPase-activating protein 32; Rho/Cdc42/Rac GTPase-activating protein RICS; RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling; p200RhoGAP; p250GAP |
| Protein Family: | Rho GTPase-activating protein |
| UniProt Gene Name: | ARHGAP32 |
| UniProt Entry Name: | RHG32_HUMAN |
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