Antibody Name:	APOBEC3D/APOBEC3F Antibody (PACO23463)
Antibody SKU:	PACO23463
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100
Species Reactivity:	Human
Immunogen:	Synthesized peptide derived from internal of human APOBEC3D/F.

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot analysis of extracts from MCF-7 cells and COS-7cells, using APOBEC3D/F antibody.
	Immunohistochemistry analysis of paraffin-embedded human placenta tissue using APOBEC3D/F antibody.

Background:

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. May inhibit the mobility of LTR and non-LTR retrotransposons.

Synonyms:

Probable DNA dC->dU-editing enzyme APOBEC-3D; EC 3.5.4.-; APOBEC3D;

UniProt Protein Function:	APOBEC3D: Probable DNA cytidine deaminase involved in foreign DNA clearance. May provide cellular innate resistance to a specific panel of genetic invaders including endogenous retroelements and a subset of viruses. Belongs to the cytidine and deoxycytidylate deaminase family.
UniProt Protein Details:	Protein type:Hydrolase; EC 3.5.4.- Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytoplasm Molecular Function:zinc ion binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines Biological Process: negative regulation of retroviral genome replication; innate immune response; defense response to virus
UniProt Code:	Q96AK3
NCBI GenInfo Identifier:	48474596
NCBI Gene ID:
NCBI Accession:	Q96AK3.1
UniProt Secondary Accession:	Q96AK3,Q5JZ91, Q7Z2N2, Q7Z2N5, Q7Z2N6,
Molecular Weight:	46598
NCBI Full Name:	DNA dC->
UniProt Protein Name:	DNA dC->dU-editing enzyme APOBEC-3D
Protein Family:	DNA dC->dU-editing enzyme
UniProt Gene Name:	APOBEC3D
UniProt Entry Name:	ABC3D_HUMAN