Name: APAF1 Antibody (PACO06254)
SKU: PACO06254
Availability: OutOfStock

Antibody Name:	APAF1 Antibody (PACO06254)
Antibody SKU:	PACO06254
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:10000, WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:	Human
Immunogen:	Synthesized peptide derived from the C-terminal region of human Apaf-1-ALT.

Form:	Liquid
Storage Buffer:	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot analysis of Jurkat cells using Apaf-1-ALT Polyclonal Antibody.
	Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody.

Synonyms:

APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19; dJ179L10.2; likely ortholog of mouse monocyte macrophage 19

UniProt Protein Function:	APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.Protein type: ApoptosisChromosomal Location of Human Ortholog: 12q23.1Cellular Component: apoptosome; cytosolMolecular Function: nucleotide binding; protein bindingBiological Process: apoptosis; caspase activation; caspase activation via cytochrome c; nervous system development; positive regulation of apoptosis; regulation of apoptosis
UniProt Protein Details:
NCBI Summary:	This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O14727-6
NCBI GenInfo Identifier:	20141188
NCBI Gene ID:	317
NCBI Accession:	O14727.2
UniProt Secondary Accession:	O14727-6,O43297, Q7Z438, Q9BXZ6, Q9UBZ5, Q9UGN8, Q9UGN9 Q9UGP0, Q9UJ58, Q9UJ59
UniProt Related Accession:	O14727-6,O14727
Molecular Weight:	37,976 Da
NCBI Full Name:	Apoptotic protease-activating factor 1
NCBI Synonym Full Names:	apoptotic peptidase activating factor 1
NCBI Official Symbol:	APAF1
NCBI Official Synonym Symbols:	CED4; APAF-1
NCBI Protein Information:	apoptotic protease-activating factor 1
UniProt Protein Name:	Apoptotic protease-activating factor 1
UniProt Synonym Protein Names:
Protein Family:	Apoptotic protease-activating factor
UniProt Gene Name:	APAF1
UniProt Entry Name: