Antibody Name:	Anti-ZYX Antibody
Antibody SKU:	CAB2135
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ZYX (NP_001010972.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ZYX (NP_001010972.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAP RPSP AISV SVSA PAFY APQK KFGP VVAP KPKV NPFR PGDS EPPP APGA QRAQ MGRV GEIP PPPP EDFP LPPP PLAG DGDD AEGA LGGA FPPP PPPI EESF PPAP LEEE IFPS PPPP PEEE GGPE APIP PPPQ PREK VSSI DLEI DSLS SLLD DMTK NDPF KARV SSGY VPPP VATP FSSK SSTK PAAG GTAP LPPW KSPS SSQP LPQV PAPA QSQT QFHV QPQP QPKP QVQL HVQS QTQP VSLA NTQP RGPP ASSP APAP KFSP VTPK FTPV ASKF SPGA PGGS GSQP NQKL GHPE
Gene ID:	7791
Uniprot:	Q15942
Cellular Location:	Cell junction, Cytoplasm, Nucleus, cytoskeleton, focal adhesion
Calculated MW:	45kDa/61kDa
Observed MW:	78kDa

Synonyms:

ZYX, ESP-2, HED-2, zyxin

Background:

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

UniProt Protein Function:	Zyxin: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Belongs to the zyxin/ajuba family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 7q32 Cellular Component: focal adhesion; cell-cell adherens junction; integral to plasma membrane; cytoplasm; stress fiber; plasma membrane; nucleus Molecular Function:protein binding; zinc ion binding Biological Process: integrin-mediated signaling pathway; cell-cell signaling; viral reproduction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; regulation of inflammatory response; stress fiber formation; signal transduction; cell adhesion
NCBI Summary:	Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15942
NCBI GenInfo Identifier:	2497677
NCBI Gene ID:	7791
NCBI Accession:	Q15942.1
UniProt Secondary Accession:	Q15942,Q6I9S4, A4D2G6, B4DQX7,
UniProt Related Accession:	Q15942
Molecular Weight:	572
NCBI Full Name:	Zyxin
NCBI Synonym Full Names:	zyxin
NCBI Official Symbol:	ZYX
NCBI Official Synonym Symbols:	ESP-2; HED-2
NCBI Protein Information:	zyxin; zyxin-2
UniProt Protein Name:	Zyxin
UniProt Synonym Protein Names:	Zyxin-2
Protein Family:	Zyxin
UniProt Gene Name:	ZYX
UniProt Entry Name:	ZYX_HUMAN

	Western blot analysis of extracts of HeLa cells, using ZYX antibody (CAB2135). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse spleen using ZYX antibody (CAB2135) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using ZYX antibody (CAB2135) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Raw264.7 cells using ZYX antibody (CAB2135) at dilution of 1:100. Blue: DAPI for nuclear staining.