Cell Cycle Antibodies 1
Anti-ZWINT Antibody (CAB6328)
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- SKU:
- CAB6328
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-ZWINT Antibody |
| Antibody SKU: | CAB6328 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human ZWINT (NP_008988.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, SW620, HepG2, Jurkat, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human ZWINT (NP_008988.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEAA ETEA EAAA LEVL AEVA GILE PVGL QEEA ELPA KILV EFVV DSQK KDKL LCSQ LQVA DFLQ NILA QEDT AKGL DPLA SEDT SRQK AIAA KEQW KELK ATYR EHVE AIKI GLTK ALTQ MEEA QRKR TQLR EAFE QLQA KKQM AMEK RRAV QNQW QLQQ EKHL QHLA EVSA EVRE RKTG TQQE LDRV FQKL GNLK QQAE QERD KLQR YQTF LQLL YTLQ GKLL FPEA EAEA ENLP DDKP QQPT RPQE QSTG DTMG RDPG VSFK AVGL QPAG DVNL P |
| Gene ID: | 11130 |
| Uniprot: | O95229 |
| Cellular Location: | Chromosome, Nucleus, centromere, kinetochore |
| Calculated MW: | 25kDa/31kDa |
| Observed MW: | 39kDa |
| Synonyms: | ZWINT, HZwint-1, KNTC2AP, SIP30, ZWINT1 |
| Background: | This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q21-q22 Cellular Component: cytoplasm; cytosol; kinetochore Molecular Function:protein binding; protein N-terminus binding Biological Process: establishment of cellular localization; mitotic cell cycle checkpoint; mitotic sister chromatid segregation; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95229 |
| NCBI GenInfo Identifier: | 117949386 |
| NCBI Gene ID: | 11130 |
| NCBI Accession: | O95229.2 |
| UniProt Secondary Accession: | O95229,Q0D2I3, Q9BWD0, A6NNV6, |
| UniProt Related Accession: | O95229 |
| Molecular Weight: | 25,544 Da |
| NCBI Full Name: | ZW10 interactor |
| NCBI Synonym Full Names: | ZW10 interacting kinetochore protein |
| NCBI Official Symbol: | ZWINT |
| NCBI Official Synonym Symbols: | SIP30; ZWINT1; KNTC2AP; HZwint-1 |
| NCBI Protein Information: | ZW10 interactor |
| UniProt Protein Name: | ZW10 interactor |
| UniProt Synonym Protein Names: | ZW10-interacting protein 1; Zwint-1 |
| Protein Family: | ZW10 interactor |
| UniProt Gene Name: | ZWINT |
| UniProt Entry Name: | ZWINT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZWINT antibody (CAB6328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of C6 cells using ZWINT Polyclonal Antibody (CAB6328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using ZWINT Polyclonal Antibody (CAB6328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using ZWINT Polyclonal Antibody (CAB6328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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