Cell Cycle Antibodies 1
Anti-ZWILCH Antibody (CAB14627)
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- SKU:
- CAB14627
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-ZWILCH Antibody |
| Antibody SKU: | CAB14627 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 292-591 of human ZWILCH (NP_060445.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | DU145, HeLa, PC-12, MCF7, Mouse brain, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 292-591 of human ZWILCH (NP_060445.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AKSA VELV QEFL NDLN KLDG FGDS TKKD TEVE TLKH DTAA VDRS VKRL FKVR SDLD FAEQ LWCK MSSS VISY QDLV KCFT LIIQ SLQR GDIQ PWLH SGSN SLLS KLIH QSYH GTMD TVSL SGTI PVQM LLEI GLDK LKKD YISF FIGQ ELAS LNHL EYFI APSV DIQE QVYR VQKL HHIL EILV SCMP FIKS QHEL LFSL TQIC IKYY KQNP LDEQ HIFQ LPVR PTAV KNLY QSEK PQKW RVEI YSGQ KKIK TVWQ LSDS SPID HLNF HKPD FSEL TLNG SLEE RIFF TNMV TCSQ VHFK |
| Gene ID: | 55055 |
| Uniprot: | Q9H900 |
| Cellular Location: | Chromosome, centromere, kinetochore |
| Calculated MW: | 54kDa/67kDa |
| Observed MW: | 70kDa |
| Synonyms: | ZWILCH, KNTC1AP, hZwilch |
| Background: |
| UniProt Protein Function: | ZWILCH: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Belongs to the ZWILCH family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 15q22.31 Cellular Component: cytosol; kinetochore Molecular Function:protein binding Biological Process: mitotic cell cycle checkpoint; sister chromatid cohesion |
| UniProt Code: | Q9H900 |
| NCBI GenInfo Identifier: | 166228729 |
| NCBI Gene ID: | 55055 |
| NCBI Accession: | Q9H900.2 |
| UniProt Secondary Accession: | Q9H900,Q6N049, Q8N404, Q96SY7, Q9NWG7, B3KVB8, |
| UniProt Related Accession: | Q9H900 |
| Molecular Weight: | 54,224 Da |
| NCBI Full Name: | Protein zwilch homolog |
| NCBI Synonym Full Names: | zwilch kinetochore protein |
| NCBI Official Symbol: | ZWILCH |
| NCBI Official Synonym Symbols: | KNTC1AP; hZwilch |
| NCBI Protein Information: | protein zwilch homolog |
| UniProt Protein Name: | Protein zwilch homolog |
| Protein Family: | Protein zwilch |
| UniProt Gene Name: | ZWILCH |
| UniProt Entry Name: | ZWILC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZWILCH antibody (CAB14627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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