Epigenetics & Nuclear Signaling Antibodies 4

Anti-ZRANB3 Antibody (CAB9555)

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SKU:
CAB9555
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ZRANB3 Antibody
Antibody SKU:CAB9555
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 818-1077 of human ZRANB3 (NP_001273497.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Raji, Jurkat, 293T, Mouse liver, Mouse heart, Mouse brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 818-1077 of human ZRANB3 (NP_001273497.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ITKQ QTKQ NCTK RYIT KEDV AVAS MDKV KNVG GHVR LITK ESRP RDPF TKKL LEDG ACVP FLNP YTVQ ADLT VKPS TSKG YLQA VDNE GNPL CLRC QQPT CQTK QACK ANSW DSRF CSLK CQEE FWIR SNNS YLRA KVFE TEHG VCQL CNVN AQEL FLRL RDAP KSQR KNLL YATW TSKL PLEQ LNEM IRNP GEGH FWQV DHIK PVYG GGGQ CSLD NLQT LCTV CHKE RTAR QAKE RSQV RRQS LASK HGSD ITRF LVKK
Gene ID:84083
Uniprot:Q5FWF4
Cellular Location:Chromosome, Nucleus
Calculated MW:42kDa/49kDa/67kDa/123kDa
Observed MW:123kDa
Synonyms:ZRANB3, 4933425L19Rik, AH2
Background:
UniProt Protein Function:ZRANB3: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks. Belongs to the SNF2/RAD54 helicase family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Helicase; EC 3.6.4.-; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: nuclear replication fork

Molecular Function:protein binding; DNA helicase activity; DNA binding; zinc ion binding; ATP binding; endodeoxyribonuclease activity

Biological Process: negative regulation of DNA recombination; DNA strand renaturation; replication fork protection; DNA repair; DNA catabolic process, endonucleolytic; response to DNA damage stimulus; replication fork processing; response to UV

UniProt Code:Q5FWF4
NCBI GenInfo Identifier:557440797
NCBI Gene ID:84083
NCBI Accession:NP_001273497.1
UniProt Secondary Accession:Q5FWF4,Q53SM1, Q6P2C4, Q8N1P4, Q9H0E8, B3KYA1, B4E375 B5MDI3, D3DP76, E9PBP0,
UniProt Related Accession:Q5FWF4
Molecular Weight:42,179 Da
NCBI Full Name:DNA annealing helicase and endonuclease ZRANB3 isoform 2
NCBI Synonym Full Names:zinc finger, RAN-binding domain containing 3
NCBI Official Symbol:ZRANB3  
NCBI Official Synonym Symbols:AH2; 4933425L19Rik  
NCBI Protein Information:DNA annealing helicase and endonuclease ZRANB3
UniProt Protein Name:DNA annealing helicase and endonuclease ZRANB3
UniProt Synonym Protein Names:Annealing helicase 2; AH2; Zinc finger Ran-binding domain-containing protein 3Including the following 2 domains:DNA annealing helicase ZRANB3 (EC:3.6.4.-)Endonuclease ZRANB3 (EC:3.1.-.-)
Protein Family:DNA annealing helicase and endonuclease
UniProt Gene Name:ZRANB3  
UniProt Entry Name:ZRAB3_HUMAN
Anti-ZRANB3 Polyclonal Antibody (CAB9555)Western blot analysis of extracts of various cell lines, using ZRANB3 antibody (CAB9555) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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