Signal Transduction Antibodies 2

Anti-ZNRF1 Antibody (CAB15918)

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SKU:
CAB15918
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ZNRF1 Antibody
Antibody SKU:CAB15918
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ZNRF1 (NP_115644.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, Jurkat, Mouse thymus, Mouse lung, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ZNRF1 (NP_115644.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGGK QSTA ARSR GPFP GVST DDSA VPPP GGAP HFGH YRTG GGAM GLRS RSVS SVAG MGMD PSTA GGVP FGLY TPAS RGTG DSER APGG GGSA SDST YAHG NGYQ ETGG GHHR DGML YLGS RASL ADAL PLHI APRW FSSH SGFK CPIC SKSV ASDE MEMH FIMC LSKP RL
Gene ID:84937
Uniprot:Q8ND25
Cellular Location:Cytoplasmic vesicle, Endosome, Lysosome, Membrane, Peripheral membrane protein, secretory vesicle, synaptic vesicle membrane
Calculated MW:23kDa/29kDa
Observed MW:24kDa
Synonyms:ZNRF1, NIN283
Background:This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons.
UniProt Protein Function:ZNRF1: E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable). 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytosol; endosome; membrane

Molecular Function:protein binding; ubiquitin-protein ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination

NCBI Summary:This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]
UniProt Code:Q8ND25
NCBI GenInfo Identifier:126253846
NCBI Gene ID:84937
NCBI Accession:Q8ND25.2
UniProt Secondary Accession:Q8ND25,Q9H083, D3DUJ9,
UniProt Related Accession:Q8ND25
Molecular Weight:29,777 Da
NCBI Full Name:E3 ubiquitin-protein ligase ZNRF1
NCBI Synonym Full Names:zinc and ring finger 1, E3 ubiquitin protein ligase
NCBI Official Symbol:ZNRF1  
NCBI Official Synonym Symbols:NIN283  
NCBI Protein Information:E3 ubiquitin-protein ligase ZNRF1
UniProt Protein Name:E3 ubiquitin-protein ligase ZNRF1
UniProt Synonym Protein Names:Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:ZNRF1  
UniProt Entry Name:ZNRF1_HUMAN
Anti-ZNRF1 Antibody (CAB15918)Western blot analysis of extracts of various cell lines, using ZNRF1 antibody (CAB15918) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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