Developmental Biology

Anti-ZIC3 Antibody (CAB9476)

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SKU:
CAB9476
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-ZIC3 Antibody
Antibody SKU:CAB9476
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZIC3 (NP_003404.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZIC3 (NP_003404.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTML LDGG PQFP GLGV GSFG APRH HEMP NREP AGMG LNPF GDST HAAA AAAA AAAF KLSP AAAH DLSS GQSS AFTP QGSG YANA LGHH HHHH HHHH HTSQ VPSY GGAA SAAF NSTR EFLF RQRS SGLS EAAS GGGQ HGLF AGSA SSLH APAG IPEP PSYL LFPG LHEQ GAGH PSPT GHVD NNQV HLGL RGEL FGRA DPYR PVAS PRTD PYAA GAQF PNYS PMNM NMGV NVAA HHGP GAFF
Gene ID:7547
Uniprot:O60481
Cellular Location:Cytoplasm, Nucleus
Calculated MW:50kDa
Observed MW:51kDa
Synonyms:ZIC3, HTX, HTX1, VACTERLX, ZNF203
Background:This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.
UniProt Protein Function:ZIC3: Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Defects in ZIC3 are the cause of visceral heterotaxy X- linked type 1 (HTX1). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy X- linked type 1 include dextrocardia, corrected transposition of great arteries, ventricular septal defect, patent ductus arteriosus, pulmonic stenosis, situs inversus viscerum, and asplenia and/or polysplenia. Defects in ZIC3 are a cause of VACTERL association X- linked with or without hydrocephalus (VACTERLX). A syndrome characterized by vertebral anomalies, anal atresia, cardiac malformations, tracheoesophageal fistula, renal anomalies (urethral atresia with hydronephrosis), and limb anomalies (hexadactyly, humeral hypoplasia, radial aplasia, and proximally placed thumb). Some patients may have hydrocephalus. Some cases of VACTERL-H are associated with increased chromosome breakage and rearrangement. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: Xq26.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: determination of left/right symmetry; heart looping; lung development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; somatic stem cell maintenance

Disease: Heterotaxy, Visceral, 1, X-linked; Vacterl Association, X-linked, With Or Without Hydrocephalus

NCBI Summary:This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq, Jul 2008]
UniProt Code:O60481
NCBI GenInfo Identifier:6137314
NCBI Gene ID:7547
NCBI Accession:O60481.1
UniProt Secondary Accession:O60481,Q14DE5, Q5JY75, B2CNW4,
UniProt Related Accession:O60481
Molecular Weight:50,045 Da
NCBI Full Name:Zinc finger protein ZIC 3
NCBI Synonym Full Names:Zic family member 3
NCBI Official Symbol:ZIC3  
NCBI Official Synonym Symbols:HTX; HTX1; ZNF203; VACTERLX  
NCBI Protein Information:zinc finger protein ZIC 3
UniProt Protein Name:Zinc finger protein ZIC 3
UniProt Synonym Protein Names:Zinc finger protein 203; Zinc finger protein of the cerebellum 3
Protein Family:Zinc finger protein
UniProt Gene Name:ZIC3  
UniProt Entry Name:ZIC3_HUMAN
Anti-ZIC3 Polyclonal Antibody (CAB9476)Western blot analysis of extracts of various cell lines, using ZIC3 antibody (CAB9476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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