Name: Anti-ZFYVE27 Antibody (CAB15943)
Brand: Cell Biology Antibodies 6
SKU: CAB15943
Availability: OutOfStock

Antibody Name:	Anti-ZFYVE27 Antibody
Antibody SKU:	CAB15943
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 217-411 of human ZFYVE27 (NP_653189.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	DU145

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 217-411 of human ZFYVE27 (NP_653189.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VEFF RVVS EYRA SLQQ RMNP KQEE HAFE SPPP PDVG GKDG LMDS TPAL TPTE DLTP GSVE EAEE AEPD EEFK DAIE ETHL VVLE DDEG APCP AEDE LALQ DNGF LSKN EVLR SKVS RLTE RLRK RYPT NNFG NCTG CSAT FSVL KKRR SCSN CGNS FCSR CCSF KVPK SSMG ATAP EAQR ETVF VCAS CNQT LSK
Gene ID:	118813
Uniprot:	Q5T4F4
Cellular Location:	Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, Recycling endosome membrane, growth cone membrane
Calculated MW:	31kDa/34kDa/35kDa/41kDa/45kDa/46kDa
Observed MW:	46kDa

Synonyms:	ZFYVE27, PROTRUDIN, SPG33, protrudin
Background:	This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear.

UniProt Protein Function:	ZFYVE27: Functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Involved in nerve growth factor-induced neurite formation. May have a more general role in cell projections formation. Defects in ZFYVE27 are the cause of spastic paraplegia autosomal dominant type 33 (SPG33). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. According to PubMed:18606302, the properties of the variant Val-191 and its frequency in some populations raise doubts on the implication of that gene in the disease. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 10q24.2 Cellular Component: axon; dendrite; endoplasmic reticulum; recycling endosome membrane Molecular Function:protein binding Biological Process: nerve growth factor receptor signaling pathway; neurite development Disease: Spastic Paraplegia 33, Autosomal Dominant
NCBI Summary:	This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]
UniProt Code:	Q5T4F4
NCBI GenInfo Identifier:	74744927
NCBI Gene ID:	118813
NCBI Accession:	Q5T4F4.1
UniProt Secondary Accession:	Q5T4F4,Q5T4F1, Q5T4F2, Q5T4F3, Q8N1K0, Q8N6D6, B7Z3S0 B7Z404, B7Z626, G8JLC3, G8JLF0, J3KP98,
UniProt Related Accession:	Q5T4F4
Molecular Weight:	41,417 Da
NCBI Full Name:	Protrudin
NCBI Synonym Full Names:	zinc finger FYVE-type containing 27
NCBI Official Symbol:	ZFYVE27
NCBI Official Synonym Symbols:	SPG33; PROTRUDIN
NCBI Protein Information:	protrudin
UniProt Protein Name:	Protrudin
UniProt Synonym Protein Names:	Zinc finger FYVE domain-containing protein 27
Protein Family:	Protrudin
UniProt Gene Name:	ZFYVE27
UniProt Entry Name:	ZFY27_HUMAN

Western blot analysis of extracts of DU145 cells, using ZFYVE27 antibody (CAB15943) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

Anti-ZFYVE27 Antibody (CAB15942)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)