Epigenetics & Nuclear Signaling Antibodies 2

Anti-ZEB2 Antibody (CAB5705)

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SKU:
CAB5705
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ZEB2 Antibody
Antibody SKU:CAB5705
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1050-1214 of human ZEB2 (NP_055610.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1050-1214 of human ZEB2 (NP_055610.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SGEK PYQC DKCG KRFS HSGS YSQH MNHR YSYC KREA EERE AAER EARE KGHL EPTE LLMN RAYL QSIT PQGY SDSE ERES MPRD GESE KEHE KEGE DGYG KLGR QDGD EEFE EEEE ESEN KSMD TDPE TIRD EEET GDHS MDDS SEDG KMET KSDH EEDN MEDG M
Gene ID:9839
Uniprot:O60315
Cellular Location:Nucleus
Calculated MW:133kDa/136kDa
Observed MW:126kDa
Synonyms:ZEB2, HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B
Background:The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: nucleus

Molecular Function:protein binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; pigmentation during development; positive regulation of melanin biosynthetic process; positive regulation of melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter

Disease: Mowat-wilson Syndrome

NCBI Summary:The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010]
UniProt Code:O60315
NCBI GenInfo Identifier:13124503
NCBI Gene ID:9839
NCBI Accession:O60315.1
UniProt Secondary Accession:O60315,Q9UED1, A0JP09, B7Z2P2, F5H814,
UniProt Related Accession:O60315
Molecular Weight:133,806 Da
NCBI Full Name:Zinc finger E-box-binding homeobox 2
NCBI Synonym Full Names:zinc finger E-box binding homeobox 2
NCBI Official Symbol:ZEB2  
NCBI Official Synonym Symbols:SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1  
NCBI Protein Information:zinc finger E-box-binding homeobox 2
UniProt Protein Name:Zinc finger E-box-binding homeobox 2
UniProt Synonym Protein Names:Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1b
Protein Family:Zinc finger E-box-binding homeobox
UniProt Gene Name:ZEB2  
UniProt Entry Name:ZEB2_HUMAN
Anti-ZEB2 Antibody (CAB5705)Western blot analysis of extracts of HepG2 cells, using ZEB2 antibody (CAB5705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Anti-ZEB2 Antibody (CAB5705)Immunohistochemistry of paraffin-embedded rat brain using ZEB2 Rabbit pAb (CAB5705) at dilution of 1:100 (40x lens).
Anti-ZEB2 Antibody (CAB5705)Immunohistochemistry of paraffin-embedded mouse brain using ZEB2 Rabbit pAb (CAB5705) at dilution of 1:100 (40x lens).
Anti-ZEB2 Antibody (CAB5705)Immunofluorescence analysis of C6 cells using ZEB2 antibody (CAB5705) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ZEB2 Antibody (CAB5705)Immunofluorescence analysis of NIH/3T3 cells using ZEB2 antibody (CAB5705) at dilution of 1:100. Blue: DAPI for nuclear staining.
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