Developmental Biology
Anti-ZEB1 Antibody (CAB16981)
- SKU:
- CAB16981
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-ZEB1 Antibody |
| Antibody SKU: | CAB16981 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human ZEB1. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat |
| Immunogen: | A synthetic peptide of human ZEB1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6935 |
| Uniprot: | P37275 |
| Cellular Location: | |
| Calculated MW: | 116kDa/122kDa/124kDa |
| Observed MW: | 180kDa |
| Synonyms: | ZEB1, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A |
| Background: | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p11.2 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity; transcription corepressor activity Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6 |
| NCBI Summary: | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010] |
| UniProt Code: | P37275 |
| NCBI GenInfo Identifier: | 6166575 |
| NCBI Gene ID: | 6935 |
| NCBI Accession: | P37275.2 |
| UniProt Secondary Accession: | P37275,Q12924, Q13800, Q2KJ05, Q5T968, Q5VZ84, Q8NB68 B4DJV0, B4DUW9, E9PCM7, F5H4I8, |
| UniProt Related Accession: | P37275 |
| Molecular Weight: | 122,084 Da |
| NCBI Full Name: | Zinc finger E-box-binding homeobox 1 |
| NCBI Synonym Full Names: | zinc finger E-box binding homeobox 1 |
| NCBI Official Symbol: | ZEB1 |
| NCBI Official Synonym Symbols: | BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1 |
| NCBI Protein Information: | zinc finger E-box-binding homeobox 1; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression) |
| UniProt Protein Name: | Zinc finger E-box-binding homeobox 1 |
| UniProt Synonym Protein Names: | NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8 |
| Protein Family: | Zinc finger E-box-binding homeobox |
| UniProt Gene Name: | ZEB1 |
| UniProt Entry Name: | ZEB1_HUMAN |
 | Western blot analysis of extracts of various cells, using ZEB1 antibody (CAB16981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min. |
 | Immunohistochemistry of paraffin-embedded rat ovary using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ZEB1 antibody (CAB16981) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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