KO Validated Antibodies 1

Anti-ZEB1 Antibody (CAB1500)[KO Validated]

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SKU:
CAB1500
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-ZEB1 Antibody [KO Validated]
Antibody SKU:CAB1500
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human ZEB1
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat
Immunogen:Recombinant protein of human ZEB1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6935
Uniprot:P37275
Cellular Location:Nucleus
Calculated MW:116kDa/122kDa/124kDa
Observed MW:200KDa
Synonyms:ZEB1, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
Background:This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function:protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation

Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6

NCBI Summary:This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
UniProt Code:P37275
NCBI GenInfo Identifier:6166575
NCBI Gene ID:6935
NCBI Accession:P37275.2
UniProt Secondary Accession:P37275,Q12924, Q13800, Q2KJ05, Q5T968, Q5VZ84, Q8NB68 B4DJV0, B4DUW9, E9PCM7, F5H4I8,
UniProt Related Accession:P37275
Molecular Weight:122,084 Da
NCBI Full Name:Zinc finger E-box-binding homeobox 1
NCBI Synonym Full Names:zinc finger E-box binding homeobox 1
NCBI Official Symbol:ZEB1  
NCBI Official Synonym Symbols:BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1  
NCBI Protein Information:zinc finger E-box-binding homeobox 1; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression)
UniProt Protein Name:Zinc finger E-box-binding homeobox 1
UniProt Synonym Protein Names:NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8
Protein Family:Zinc finger E-box-binding homeobox
UniProt Gene Name:ZEB1  
UniProt Entry Name:ZEB1_HUMAN
Anti-ZEB1 Antibody [KO Validated] (CAB1500)Western blot analysis of extracts of Jurkat cells, using ZEB1 Rabbit pAb (CAB1500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-ZEB1 Antibody [KO Validated] (CAB1500)Western blot analysis of extracts from normal (control) and ZEB1 Rabbit pAb knockout (KO) HeLa cells, using ZEB1 Rabbit pAb antibody (CAB1500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-ZEB1 Antibody [KO Validated] (CAB1500)Immunohistochemistry of paraffin-embedded human gastric cancer using ZEB1 antibody (CAB1500) at dilution of 1:200 (40x lens).
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